This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029681
PrimePCR™ PreAmp for SYBR® Green Assay: COG8, Human
PrimePCR™ Template for SYBR® Green Assay: COG8, Human
This gene encodes a protein that is a component of the conserved oligomeric Golgi (COG) complex a multiprotein complex that plays a structural role in the Golgi apparatus and is involved in intracellular membrane trafficking and glycoprotein modification. Mutations in this gene cause congenital disorder of glycosylation type IIh a disease that is characterized by under-glycosylated serum proteins and whose symptoms include severe psychomotor retardation failure to thrive seizures and dairy and wheat product intolerance. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.