PrimePCR™ SYBR® Green Assay: SIM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029624

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SIM2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SIM2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014954
Assay Design:   Intron-spanning
Chromosome Location:   21:38095346-38098489question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000290399 ENST00000431229 ENST00000430056

Gene Information

SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. The Drosophila sim gene encodes a transcription factor that is a master regulator of fruit fly neurogenesis. SIM2 maps within the so-called Down syndrome chromosomal region. Based on the mapping position its potential function as transcriptional repressor and similarity to Drosophila sim it is proposed that SIM2 may contribute to some specific Down syndrome phenotypes [provided by RefSeq Jul 2008]

Gene Symbol:   SIM2
Gene Name:   single-minded homolog 2 (Drosophila)
Aliases:   MGC119447, SIM, bHLHe15
RefSeq:   NC_000021.8 NG_016193.1 NT_011512.11
Ensembl:   ENSG00000159263
Entrez:   6493
Chromosome Mapping:   21q22.2,21q22.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 36.640000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download