This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029527
PrimePCR™ PreAmp for SYBR® Green Assay: SKIL, Human
PrimePCR™ Template for SYBR® Green Assay: SKIL, Human
The protein encoded by this gene is a component of the SMAD pathway which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.