PrimePCR™ SYBR® Green Assay: C9orf116, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014225
Assay Design:   Intron-spanning
Chromosome Location:   9:138390457-138391639question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000429260 ENST00000371789 ENST00000371791

Gene Information

Description Not Available

Gene Symbol:   C9orf116
Gene Name:   chromosome 9 open reading frame 116
Aliases:   FLJ13945, MGC29761
RefSeq:   NC_000009.11 NT_019501.13
Ensembl:   ENSG00000160345
Entrez:   138162
UniGene:   Hs.414028
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.750000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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