PrimePCR™ SYBR® Green Assay: EFHD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029256

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EFHD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EFHD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014201
Assay Design:   Intron-spanning
Chromosome Location:   2:233527604-233537135question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000409613 ENST00000264059 ENST00000409708 ENST00000427698 ENST00000410095 ENST00000540187

Gene Information

EFHD1 is an EF-hand domain-containing protein that displays increased expression during neuronal differentiation (Tominaga and Tomooka 2002 [PubMed 12270117]).[supplied by OMIM Mar 2008]

Gene Symbol:   EFHD1
Gene Name:   EF-hand domain family, member D1
Aliases:   DKFZp781H0842, FLJ13612, MST133, MSTP133, SWS2
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000115468
Entrez:   80303
Chromosome Mapping:   2q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.510000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download