This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029179
PrimePCR™ PreAmp for SYBR® Green Assay: HPN, Human
PrimePCR™ Template for SYBR® Green Assay: HPN, Human
This gene encodes a type II transmembrane serine protease. The encoded protein has an extracellular region that consists of two domains a catalytic serine protease domain and a non-catalytic scavenger receptor cysteine-rich domain. This protein may be involved in diverse cellular functions including blood coagulation maintenance of cell morphology and the growth and progression of certain cancers particularly prostate cancer. Alternative splicing results in multiple transcript variants.[provided by RefSeq Apr 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.