PrimePCR™ SYBR® Green Assay: NGFR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014020
Assay Design:   Intron-spanning
Chromosome Location:   17:47583850-47587820question
Amplicon Length:   188
Splice Variants Targeted:   ENST00000172229 ENST00000504201

Gene Information

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region a single transmembrane domain and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq Jul 2008]

Gene Symbol:   NGFR
Gene Name:   nerve growth factor receptor
Aliases:   CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000064300
Entrez:   4804
UniGene:   Hs.415768
Chromosome Mapping:   17q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.960000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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