PrimePCR™ SYBR® Green Assay: VPS29, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013966
Assay Design:   Intron-spanning
Chromosome Location:   12:110930882-110933860question
Amplicon Length:   169
Splice Variants Targeted:   ENST00000549578 ENST00000360579 ENST00000552130 ENST00000546588 ENST00000549970 ENST00000397678 ENST00000447578

Gene Information

This gene belongs to a group of vacuolar protein sorting (VPS) genes that when functionally impaired disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex termed the retromer complex which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and usage of multiple polyadenylation sites have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   VPS29
Gene Name:   vacuolar protein sorting 29 homolog (S. cerevisiae)
Aliases:   DC15, DKFZp564F0223, FLJ20492, PEP11
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000111237
Entrez:   51699
UniGene:   Hs.600114
Chromosome Mapping:   12q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.160000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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