PrimePCR™ SYBR® Green Assay: CENPT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013943
Assay Design:   Intron-spanning
Chromosome Location:   16:67862621-67863382question
Amplicon Length:   153
Splice Variants Targeted:   ENST00000440851 ENST00000219172 ENST00000436104

Gene Information

The centromere is a specialized chromatin domain present throughout the cell cycle that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPT is an additional factor required for centromere assembly (Foltz et al. 2006 [PubMed 16622419]).[supplied by OMIM Mar 2008]

Gene Symbol:   CENPT
Gene Name:   centromere protein T
Aliases:   C16orf56, CENP-T, FLJ13111, FLJ43376
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000102901
Entrez:   80152
UniGene:   Hs.288382
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.290000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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