PrimePCR™ SYBR® Green Assay: ATAD3A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013890
Assay Design:   Intron-spanning
Chromosome Location:   1:1461865-1463142question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000378756 ENST00000378755 ENST00000339113 ENST00000400830 ENST00000536055 ENST00000378759

Gene Information

ATAD3A and ATAD3B (MIM 612317) are mitochondrial membrane proteins that contribute to the stabilization of large mitochondrial DNA (mtDNA)-protein complexes called nucleoids (He et al. 2007 [PubMed 17210950]).[supplied by OMIM Sep 2008]

Gene Symbol:   ATAD3A
Gene Name:   ATPase family, AAA domain containing 3A
Aliases:   FLJ10709, FLJ35514
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000197785
Entrez:   55210
UniGene:   Hs.23413
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.310000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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