This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: HCRT, Human
PrimePCR™ Template for SYBR® Green Assay: HCRT, Human
This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides orexin A and orexin B by proteolytic processing. Orexin A and orexin B which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2 function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior metabolism and homeostasis. [provided by RefSeq Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.