PrimePCR™ SYBR® Green Assay: SLC25A22, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC25A22, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC25A22, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013765
Assay Design:   Intron-spanning
Chromosome Location:   11:793529-794803question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000320230 ENST00000531214 ENST00000481290 ENST00000531437 ENST00000533385 ENST00000526152 ENST00000528606 ENST00000527723 ENST00000531514 ENST00000528936 ENST00000456706 ENST00000532484

Gene Information

This gene encodes a mitochondrial glutamate carrier. Mutations in this gene are associated with early infantile epileptic encephalopathy. Multiple alternatively spliced variants encoding the same protein have been identified.[provided by RefSeq Jul 2010]

Gene Symbol:   SLC25A22
Gene Name:   solute carrier family 25 (mitochondrial carrier: glutamate), member 22
Aliases:   EIEE3, FLJ13044, GC1, NET44
RefSeq:   NC_000011.9 NT_009237.18 NG_023407.1
Ensembl:   ENSG00000177542
Entrez:   79751
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.600000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download