PrimePCR™ SYBR® Green Assay: FAM53A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029016

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013739
Assay Design:   Intron-spanning
Chromosome Location:   4:1659141-1670484question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000489363 ENST00000461064 ENST00000472884 ENST00000463238 ENST00000308132

Gene Information

Description Not Available

Gene Symbol:   FAM53A
Gene Name:   family with sequence similarity 53, member A
Aliases:   DNTNP
RefSeq:   NC_000004.11 NT_006051.18
Ensembl:   ENSG00000174137
Entrez:   152877
UniGene:   Hs.143314
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.000000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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