PrimePCR™ SYBR® Green Assay: SURF6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029002

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SURF6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SURF6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013709
Assay Design:   Intron-spanning
Chromosome Location:   9:136200554-136201361question
Amplicon Length:   193
Splice Variants Targeted:   ENST00000372022

Gene Information

This gene is located in the surfeit gene cluster a group of very tightly linked genes that do not share sequence similarity. The gene demonstrates features of a housekeeping gene being ubiquitously expressed and the encoded protein has been localized to the nucleolus. The protein includes motifs found in both the mouse and fish orthologs which suggests a putative function as a nucleolar-matrix protein with nucleic acid-binding properties based on characteristics determined in mouse. [provided by RefSeq Jul 2008]

Gene Symbol:   SURF6
Gene Name:   surfeit 6
Aliases:   FLJ30322, RRP14
RefSeq:   NC_000009.11 NG_000837.1 NT_035014.4 NW_003315925.1
Ensembl:   ENSG00000148296
Entrez:   6838
Chromosome Mapping:   9q34.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.140000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download