PrimePCR™ SYBR® Green Assay: MEFV, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028999

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013702
Assay Design:   Intron-spanning
Chromosome Location:   16:3297101-3298980question
Amplicon Length:   188
Splice Variants Targeted:   ENST00000219596 ENST00000339854 ENST00000541159 ENST00000536379 ENST00000545159 ENST00000534868

Gene Information

This gene encodes a protein also known as pyrin or marenostrin that is an important modulator of innate immunity. Mutations in this gene are associated with Mediterranean fever a hereditary periodic fever syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   MEFV
Gene Name:   Mediterranean fever
Aliases:   FMF, MEF, MGC126560, MGC126586, TRIM20
RefSeq:   NC_000016.9 NG_007871.1 NT_010393.16
Ensembl:   ENSG00000103313
Entrez:   4210
UniGene:   Hs.632221
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.060000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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