PrimePCR™ SYBR® Green Assay: POLR2D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028989

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: POLR2D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: POLR2D, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013682
Assay Design:   Intron-spanning
Chromosome Location:   2:128605652-128608216question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000272645 ENST00000409698

Gene Information

This gene encodes the fourth largest subunit of RNA polymerase II the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   POLR2D
Gene Name:   polymerase (RNA) II (DNA directed) polypeptide D
Aliases:   HSRBP4, HSRPB4, RBP4, RPB16
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000144231
Entrez:   5433
Chromosome Mapping:   2q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.640000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download