PrimePCR™ SYBR® Green Assay: C5orf4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028971

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013649
Assay Design:   Intron-spanning
Chromosome Location:   5:154210399-154214420question
Amplicon Length:   176
Splice Variants Targeted:   ENST00000326080 ENST00000517938 ENST00000519501 ENST00000518651

Gene Information

Description Not Available

Gene Symbol:   C5orf4
Gene Name:   chromosome 5 open reading frame 4
Aliases:   FLJ13758
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000170271
Entrez:   10826
UniGene:   Hs.519694
Chromosome Mapping:   5q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 34.690000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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