PrimePCR™ SYBR® Green Assay: HIGD1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028952

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013620
Assay Design:   Intron-spanning
Chromosome Location:   3:42826722-42827566question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000321331 ENST00000418900 ENST00000430190

Gene Information

Description Not Available

Gene Symbol:   HIGD1A
Gene Name:   HIG1 hypoxia inducible domain family, member 1A
Aliases:   DKFZp564K247, HIG1
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000181061
Entrez:   25994
UniGene:   Hs.728813
Chromosome Mapping:   3p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.360000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download