PrimePCR™ SYBR® Green Assay: FCN3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028896

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013505
Assay Design:   Intron-spanning
Chromosome Location:   1:27697384-27699712question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000270879 ENST00000354982 ENST00000498393

Gene Information

Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum there are two types of ficolins both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants each encoding a distinct isoform have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   FCN3
Gene Name:   ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)
Aliases:   FCNH, HAKA1, MGC22543
RefSeq:   NC_000001.10 NG_016279.1 NT_004610.19
Ensembl:   ENSG00000142748
Entrez:   8547
UniGene:   Hs.333383
Chromosome Mapping:   1p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.000000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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