This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028896
PrimePCR™ PreAmp for SYBR® Green Assay: FCN3, Human
PrimePCR™ Template for SYBR® Green Assay: FCN3, Human
Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum there are two types of ficolins both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants each encoding a distinct isoform have been identified. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.