PrimePCR™ SYBR® Green Assay: ZNF185, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028888

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013486
Assay Design:   Intron-spanning
Chromosome Location:   X:152132449-152134051question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000370268 ENST00000370270 ENST00000426821 ENST00000454925 ENST00000535861 ENST00000539731 ENST00000449285 ENST00000318504 ENST00000535156 ENST00000324823 ENST00000318529

Gene Information

Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions including cell proliferation differentiation and apoptosis. This gene encodes a LIM-domain zinc finger protein. The LIM domain is composed of two contiguous zinc finger domains separated by a two-amino acid residue hydrophobic linker. The LIM domain mediates protein:protein interactions. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq May 2010]

Gene Symbol:   ZNF185
Gene Name:   zinc finger protein 185 (LIM domain)
Aliases:   Not Available
RefSeq:   NC_000023.10 NT_167198.1 NG_021255.1
Ensembl:   ENSG00000147394
Entrez:   7739
UniGene:   Hs.16622
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.020000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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