PrimePCR™ SYBR® Green Assay: FBLN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028857

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013417
Assay Design:   Intron-spanning
Chromosome Location:   3:13678036-13679166question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000404922 ENST00000295760 ENST00000492059 ENST00000535798

Gene Information

This gene encodes an extracellular matrix protein which belongs to the fibulin family. This protein binds various extracellular ligands and calcium. It may play a role during organ development in particular during the differentiation of heart skeletal and neuronal structures. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   FBLN2
Gene Name:   fibulin 2
Aliases:   Not Available
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000163520
Entrez:   2199
UniGene:   Hs.198862
Chromosome Mapping:   3p25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.550000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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