PrimePCR™ SYBR® Green Assay: AKT1S1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: AKT1S1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AKT1S1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013399
Assay Design:   Intron-spanning
Chromosome Location:   19:50375114-50376283question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000391833 ENST00000344175 ENST00000391832 ENST00000391835 ENST00000391831 ENST00000391834

Gene Information

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH MIM 113508) when phosphorylated (Kovacina et al. 2003 [PubMed 12524439]).[supplied by OMIM Mar 2008]

Gene Symbol:   AKT1S1
Gene Name:   AKT1 substrate 1 (proline-rich)
Aliases:   Lobe, MGC2865, PRAS40
RefSeq:   NC_000019.9 NG_027717.1 NT_011109.16
Ensembl:   ENSG00000204673
Entrez:   84335
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.830000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download