PrimePCR™ SYBR® Green Assay: ZNF346, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF346, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF346, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013374
Assay Design:   Intron-spanning
Chromosome Location:   5:176489098-176491613question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000358149 ENST00000506693 ENST00000512315 ENST00000503425 ENST00000511834 ENST00000503039 ENST00000261948

Gene Information

The protein encoded by this gene is a nucleolar zinc finger protein that preferentially binds to double-stranded (ds) RNA or RNA/DNA hybrids rather than DNA alone. Mutational studies indicate that the zinc finger domains are not only essential for dsRNA binding but are also required for its nucleolar localization. The encoded protein may be involved in cell growth and survival. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNF346
Gene Name:   zinc finger protein 346
Aliases:   DKFZp547M223, JAZ, Zfp346
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000113761
Entrez:   23567
Chromosome Mapping:   5q35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.330000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download