PrimePCR™ SYBR® Green Assay: FKTN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028810

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FKTN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FKTN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013294
Assay Design:   Intron-spanning
Chromosome Location:   9:108380244-108382263question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000223528 ENST00000457847 ENST00000357998

Gene Information

The protein encoded by this gene is a putative transmembrane protein that is localized to the cis-Golgi compartment where it may be involved in the glycosylation of alpha-dystroglycan in skeletal muscle. The encoded protein is thought to be a glycosyltransferase and could play a role in brain development. Defects in this gene are a cause of Fukuyama-type congenital muscular dystrophy (FCMD) Walker-Warburg syndrome (WWS) limb-girdle muscular dystrophy type 2M (LGMD2M) and dilated cardiomyopathy type 1X (CMD1X). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Nov 2010]

Gene Symbol:   FKTN
Gene Name:   fukutin
Aliases:   CMD1X, FCMD, LGMD2M, MDDGA4, MDDGB4, MDDGC4, MGC126857, MGC134944, MGC134945, MGC138243
RefSeq:   NC_000009.11 NG_008754.1 NT_008470.19
Ensembl:   ENSG00000106692
Entrez:   2218
Chromosome Mapping:   9q31-q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.360000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download