PrimePCR™ SYBR® Green Assay: METTL15, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013292
Assay Design:   Intron-spanning
Chromosome Location:   11:28131916-28134974question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000406787 ENST00000403099 ENST00000407364 ENST00000303459 ENST00000342303 ENST00000379199

Gene Information

Description Not Available

Gene Symbol:   METTL15
Gene Name:   methyltransferase like 15
Aliases:   FLJ33979, METT5D1
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000169519
Entrez:   196074
UniGene:   Hs.243326
Chromosome Mapping:   11p14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.060000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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