PrimePCR™ SYBR® Green Assay: LMO7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028805

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LMO7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LMO7, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013275
Assay Design:   Intron-spanning
Chromosome Location:   13:76414221-76415281question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000341547 ENST00000377499 ENST00000321797 ENST00000526202 ENST00000465261 ENST00000447038 ENST00000524651 ENST00000525914 ENST00000357063 ENST00000377534

Gene Information

This gene encodes a protein containing a calponin homology (CH) domain a PDZ domain and a LIM domain and may be involved in protein-protein interactions. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene however the full-length nature of some variants is not known. [provided by RefSeq Jan 2009]

Gene Symbol:   LMO7
Gene Name:   LIM domain 7
Aliases:   FBX20, FBXO20, KIAA0858, LOMP
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000136153
Entrez:   4008
Chromosome Mapping:   13q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.560000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download