PrimePCR™ SYBR® Green Assay: CLN3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028757

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013172
Assay Design:   Intron-spanning
Chromosome Location:   16:28499912-28500706question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000359984 ENST00000360019 ENST00000354630 ENST00000355477 ENST00000357806 ENST00000357857 ENST00000395653 ENST00000357076

Gene Information

This gene encodes a protein that is involved in lysosomal function. Mutations in this as well as other neuronal ceroid-lipofuscinosis (CLN) genes cause neurodegenerative diseases commonly known as Batten disease or collectively known as neuronal ceroid lipofuscinoses (NCLs). Many alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CLN3
Gene Name:   ceroid-lipofuscinosis, neuronal 3
Aliases:   BTS, JNCL, MGC102840
RefSeq:   NC_000016.9 NT_010393.16 NG_008654.1
Ensembl:   ENSG00000188603
Entrez:   1201
UniGene:   Hs.534667
Chromosome Mapping:   16p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.820000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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