PrimePCR™ SYBR® Green Assay: SMCP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028746

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013143
Assay Design:   Intron-spanning
Chromosome Location:   1:152850868-152856936question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000368765

Gene Information

Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated flattened and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. The protein encoded by this gene localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath. [provided by RefSeq Jul 2008]

Gene Symbol:   SMCP
Gene Name:   sperm mitochondria-associated cysteine-rich protein
Aliases:   HSMCSGEN1, MCS, MCSP, MGC26305, MGC26519
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163206
Entrez:   4184
UniGene:   Hs.111850
Chromosome Mapping:   1q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.040000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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