PrimePCR™ SYBR® Green Assay: OTOA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028726

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013099
Assay Design:   Intron-spanning
Chromosome Location:   16:21728231-21730484question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000388958 ENST00000388956 ENST00000286149 ENST00000388957

Gene Information

The protein encoded by this gene is specifically expressed in the inner ear and is located at the interface between the apical surface of the inner ear sensory epithelia and their overlying acellular gels. It is prposed that this protein is involved in the attachment of the inner ear acellular gels to the apical surface of the underlying nonsensory cells. Mutations in this gene are associated with autosomal recessive deafness type 22 (DFNB22). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   OTOA
Gene Name:   otoancorin
Aliases:   CT108, DFNB22, FLJ32773, MGC157747, MGC39813
RefSeq:   NC_000016.9 NT_010393.16 NG_012973.1
Ensembl:   ENSG00000155719
Entrez:   146183
UniGene:   Hs.408336
Chromosome Mapping:   16p12.2,16p12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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