PrimePCR™ SYBR® Green Assay: TCF25, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028651

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012923
Assay Design:   Intron-spanning
Chromosome Location:   16:89972618-89973726question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000263346 ENST00000263347

Gene Information

TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development (Steen and Lindholm 2008 [PubMed 18068114]).[supplied by OMIM Sep 2008]

Gene Symbol:   TCF25
Gene Name:   transcription factor 25 (basic helix-loop-helix)
Aliases:   Hulp1, KIAA1049, NULP1, PRO2620, hKIAA1049
RefSeq:   NC_000016.9 NT_010542.15
Ensembl:   ENSG00000141002
Entrez:   22980
UniGene:   Hs.415342
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.960000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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