PrimePCR™ SYBR® Green Assay: ADSSL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028640

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012895
Assay Design:   Intron-spanning
Chromosome Location:   14:105209448-105211175question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000330877 ENST00000332972

Gene Information

ADSSL1 is a muscle isozyme of adenylosuccinate synthase (EC 6.3.4.4) which catalyzes the initial reaction in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP) (Sun et al. 2005 [PubMed 15786719]).[supplied by OMIM Dec 2008]

Gene Symbol:   ADSSL1
Gene Name:   adenylosuccinate synthase like 1
Aliases:   FLJ38602
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000185100
Entrez:   122622
UniGene:   Hs.592327
Chromosome Mapping:   14q32.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.260000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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