This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028624
PrimePCR™ PreAmp for SYBR® Green Assay: ALDOB, Human
PrimePCR™ Template for SYBR® Green Assay: ALDOB, Human
Fructose-16-bisphosphate aldolase (EC 18.104.22.168) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-16-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A B and C are distinct proteins the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver kidney and intestine aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq Dec 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.