PrimePCR™ SYBR® Green Assay: CA14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012822
Assay Design:   Intron-spanning
Chromosome Location:   1:150233990-150234685question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000369111

Gene Information

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes including respiration calcification acid-base balance bone resorption and the formation of aqueous humor cerebrospinal fluid saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform CA XII; however they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq Jul 2008]

Gene Symbol:   CA14
Gene Name:   carbonic anhydrase XIV
Aliases:   CAXiV
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000118298
Entrez:   23632
UniGene:   Hs.528988
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.100000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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