PrimePCR™ SYBR® Green Assay: FAM83D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028576

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012742
Assay Design:   Intron-spanning
Chromosome Location:   20:37570654-37576578question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000217429 ENST00000424027

Gene Information

Description Not Available

Gene Symbol:   FAM83D
Gene Name:   family with sequence similarity 83, member D
Aliases:   C20orf129, CHICA, FLJ38341, dJ616B8.3
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101447
Entrez:   81610
UniGene:   Hs.726442
Chromosome Mapping:   20q11.22-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.710000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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