PrimePCR™ SYBR® Green Assay: APOA4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028570

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012731
Assay Design:   Intron-spanning
Chromosome Location:   11:116692551-116693464question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000357780

Gene Information

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis the intestine in association with chylomicron particles. Although its precise function is not known apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq Jul 2008]

Gene Symbol:   APOA4
Gene Name:   apolipoprotein A-IV
Aliases:   MGC142154, MGC142156
RefSeq:   NC_000011.9 NG_012044.1 NT_033899.8
Ensembl:   ENSG00000110244
Entrez:   337
UniGene:   Hs.1247
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.410000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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