PrimePCR™ SYBR® Green Assay: CCDC80, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028521

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012615
Assay Design:   Intron-spanning
Chromosome Location:   3:112326048-112328922question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000206423 ENST00000439685 ENST00000479368

Gene Information

Description Not Available

Gene Symbol:   CCDC80
Gene Name:   coiled-coil domain containing 80
Aliases:   DRO1, MGC131805, MGC134851, SSG1, URB, okuribin
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000091986
Entrez:   151887
UniGene:   Hs.477128
Chromosome Mapping:   3q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.960000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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