PrimePCR™ SYBR® Green Assay: APLP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028482

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: APLP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: APLP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012534
Assay Design:   Intron-spanning
Chromosome Location:   11:129998994-129999979question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000528499 ENST00000263574 ENST00000345598 ENST00000338167 ENST00000278756 ENST00000543137 ENST00000539648

Gene Information

This gene encodes amyloid precursor- like protein 2 (APLP2) which is a member of the APP (amyloid precursor protein) family including APP APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin- copper- and zinc- binding domains at the N-terminus BPTI/Kunitz inhibitor and E2 domains in the middle region and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Aug 2011]

Gene Symbol:   APLP2
Gene Name:   amyloid beta (A4) precursor-like protein 2
Aliases:   APLP-2, APPH, APPL2, CDEBP
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000084234
Entrez:   334
Chromosome Mapping:   11q23-q25,11q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 34.780000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download