This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028482
PrimePCR™ PreAmp for SYBR® Green Assay: APLP2, Human
PrimePCR™ Template for SYBR® Green Assay: APLP2, Human
This gene encodes amyloid precursor- like protein 2 (APLP2) which is a member of the APP (amyloid precursor protein) family including APP APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin- copper- and zinc- binding domains at the N-terminus BPTI/Kunitz inhibitor and E2 domains in the middle region and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.