This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028473
PrimePCR™ PreAmp for SYBR® Green Assay: CDKN1B, Human
PrimePCR™ Template for SYBR® Green Assay: CDKN1B, Human
This gene encodes a cyclin-dependent kinase inhibitor which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes and thus controls the cell cycle progression at G1. The degradation of this protein which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.