PrimePCR™ SYBR® Green Assay: ST7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012500
Assay Design:   Intron-spanning
Chromosome Location:   7:116830917-116849914question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000393446 ENST00000265437 ENST00000393451 ENST00000323984 ENST00000393449 ENST00000446490 ENST00000432298 ENST00000422922 ENST00000393443 ENST00000393447 ENST00000393444 ENST00000490039

Gene Information

The gene for this product maps to a region on chromosome 7 identified as an autism-susceptibility locus. Mutation screening of the entire coding region in autistic individuals failed to identify phenotype-specific variants suggesting that coding mutations for this gene are unlikely to be involved in the etiology of autism. The function of this gene product has not been determined. Transcript variants encoding different isoforms of this protein have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   ST7
Gene Name:   suppression of tumorigenicity 7
Aliases:   DKFZp762O2113, ETS7q, FAM4A, FAM4A1, HELG, RAY1, SEN4, TSG7
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000004866
Entrez:   7982
UniGene:   Hs.368131
Chromosome Mapping:   7q31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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