PrimePCR™ SYBR® Green Assay: VPS37C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028449

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VPS37C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VPS37C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012453
Assay Design:   Intron-spanning
Chromosome Location:   11:60900759-60901631question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000301765 ENST00000538036 ENST00000540084

Gene Information

VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I) a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al. 2005 [PubMed 15509564]).[supplied by OMIM Mar 2008]

Gene Symbol:   VPS37C
Gene Name:   vacuolar protein sorting 37 homolog C (S. cerevisiae)
Aliases:   FLJ20847
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000167987
Entrez:   55048
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994600
y-intercept 37.790000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download