PrimePCR™ SYBR® Green Assay: PHF15, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028428

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012400
Assay Design:   Intron-spanning
Chromosome Location:   5:133900194-133901849question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000282605 ENST00000361895 ENST00000402835 ENST00000395003 ENST00000448712 ENST00000413974 ENST00000432594

Gene Information

Description Not Available

Gene Symbol:   PHF15
Gene Name:   PHD finger protein 15
Aliases:   JADE2, KIAA0239
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000043143
Entrez:   23338
UniGene:   Hs.732155
Chromosome Mapping:   5q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.550000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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