PrimePCR™ SYBR® Green Assay: LETM2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012369
Assay Design:   Intron-spanning
Chromosome Location:   8:38261989-38264892question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000297720 ENST00000524874 ENST00000379957 ENST00000523983 ENST00000527710

Gene Information

Description Not Available

Gene Symbol:   LETM2
Gene Name:   leucine zipper-EF-hand containing transmembrane protein 2
Aliases:   FLJ25409
RefSeq:   NG_023292.1 NC_000008.10 NT_167187.1
Ensembl:   ENSG00000165046
Entrez:   137994
UniGene:   Hs.696457
Chromosome Mapping:   8p11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 33.680000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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