PrimePCR™ SYBR® Green Assay: ANKRD44, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028413

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012361
Assay Design:   Intron-spanning
Chromosome Location:   2:197987490-197990597question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000328737 ENST00000409153 ENST00000409919 ENST00000282272 ENST00000450567 ENST00000337207 ENST00000539527

Gene Information

Description Not Available

Gene Symbol:   ANKRD44
Gene Name:   ankyrin repeat domain 44
Aliases:   FLJ11570, MGC21968, MGC70444, PP6-ARS-B
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000065413
Entrez:   91526
Chromosome Mapping:   2q33.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 35.410000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download