This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028404
PrimePCR™ PreAmp for SYBR® Green Assay: TIAL1, Human
PrimePCR™ Template for SYBR® Green Assay: TIAL1, Human
The protein encoded by this gene is a member of a family of RNA-binding proteins has three RNA recognition motifs (RRMs) and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control splicing and apoptosis. Alternate transcriptional splice variants encoding different isoforms have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.