PrimePCR™ SYBR® Green Assay: TIAL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028404

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012342
Assay Design:   Intron-spanning
Chromosome Location:   10:121335198-121336179question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000369093 ENST00000436547 ENST00000369092

Gene Information

The protein encoded by this gene is a member of a family of RNA-binding proteins has three RNA recognition motifs (RRMs) and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control splicing and apoptosis. Alternate transcriptional splice variants encoding different isoforms have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. [provided by RefSeq Jul 2008]

Gene Symbol:   TIAL1
Gene Name:   TIA1 cytotoxic granule-associated RNA binding protein-like 1
Aliases:   MGC33401, TCBP, TIAR
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000151923
Entrez:   7073
UniGene:   Hs.501203
Chromosome Mapping:   10q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.390000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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