PrimePCR™ SYBR® Green Assay: NUDT5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028402

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NUDT5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NUDT5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012340
Assay Design:   Intron-spanning
Chromosome Location:   10:12214804-12219810question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000491614 ENST00000378929 ENST00000378937 ENST00000378940 ENST00000378927 ENST00000537776

Gene Information

Nudix hydrolases such as NUDT5 eliminate toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites (McLennan 1999 [PubMed 10373642]).[supplied by OMIM Mar 2008]

Gene Symbol:   NUDT5
Gene Name:   nudix (nucleoside diphosphate linked moiety X)-type motif 5
Aliases:   YSA1, YSA1H, hYSAH1
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000165609
Entrez:   11164
Chromosome Mapping:   10p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.840000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download