PrimePCR™ SYBR® Green Assay: RBM5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028397

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012331
Assay Design:   Intron-spanning
Chromosome Location:   3:50144951-50145668question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000347869 ENST00000536082 ENST00000543047 ENST00000539538 ENST00000544851

Gene Information

This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3 and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer. [provided by RefSeq Oct 2011]

Gene Symbol:   RBM5
Gene Name:   RNA binding motif protein 5
Aliases:   FLJ39876, G15, LUCA15, RMB5
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000003756
Entrez:   10181
UniGene:   Hs.439480
Chromosome Mapping:   3p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 34.140000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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