PrimePCR™ SYBR® Green Assay: CCDC129, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028352

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012233
Assay Design:   Intron-spanning
Chromosome Location:   7:31683452-31690793question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000319386 ENST00000407970 ENST00000409210 ENST00000451887 ENST00000538406

Gene Information

Description Not Available

Gene Symbol:   CCDC129
Gene Name:   coiled-coil domain containing 129
Aliases:   FLJ38344
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000180347
Entrez:   223075
UniGene:   Hs.224269
Chromosome Mapping:   7p14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.720000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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