PrimePCR™ SYBR® Green Assay: PION, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028349

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PION, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PION, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012228
Assay Design:   Intron-spanning
Chromosome Location:   7:76958672-76959676question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000257626 ENST00000415112

Gene Information

Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311) a protease with numerous substrates. PION or GSAP selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al. 2010 [PubMed 20811458]).[supplied by OMIM Nov 2010]

Gene Symbol:   PION
Gene Name:   pigeon homolog (Drosophila)
Aliases:   DKFZp667B242, GSAP, MGC126548
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000186088
Entrez:   54103
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.700000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download