This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028349
PrimePCR™ PreAmp for SYBR® Green Assay: PION, Human
PrimePCR™ Template for SYBR® Green Assay: PION, Human
Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311) a protease with numerous substrates. PION or GSAP selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al. 2010 [PubMed 20811458]).[supplied by OMIM Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.