PrimePCR™ SYBR® Green Assay: C21orf59, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012214
Assay Design:   Intron-spanning
Chromosome Location:   21:33976555-33982191question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000431216 ENST00000553001 ENST00000440966 ENST00000382549 ENST00000290155 ENST00000458138 ENST00000543202

Gene Information

Description Not Available

Gene Symbol:   C21orf59
Gene Name:   chromosome 21 open reading frame 59
Aliases:   C21orf48, FLJ20467, FLJ37137, FLJ40247
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000159079
Entrez:   56683
UniGene:   Hs.5811
Chromosome Mapping:   21q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.140000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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