PrimePCR™ SYBR® Green Assay: PTPRU, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012141
Assay Design:   Intron-spanning
Chromosome Location:   1:29586505-29587210question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000345512 ENST00000373779 ENST00000428026 ENST00000460170 ENST00000356870 ENST00000323874

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This PTP possesses an extracellular region a single transmembrane region and two tandem intracellular catalytic domains and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP (MAM) domain Ig-like and fibronectin type III-like repeats. This PTP was thought to play roles in cell-cell recognition and adhesion. Studies of the similar gene in mice suggested the role of this PTP in early neural development. The expression of this gene was reported to be regulated by phorbol myristate acetate (PMA) or calcium ionophore in Jurkat T lymphoma cells. Alternatively spliced transcript variants have been reported. [provided by RefSeq Aug 2010]

Gene Symbol:   PTPRU
Gene Name:   protein tyrosine phosphatase, receptor type, U
Aliases:   FLJ37530, FMI, GLEPP1, PCP-2, PTP, PTP-J, PTP-PI, PTPPSI, PTPRO, PTPU2, R-PTP-PSI, hPTP-J
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000060656
Entrez:   10076
UniGene:   Hs.19718
Chromosome Mapping:   1p35.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994600
y-intercept 36.000000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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